Unfortunately, RDTs are ineffective in detecting P. knowlesi and asymptomatic malaria with a low number of parasites (under approximately 200 parasites/μl), may produce false-positive results even after resolution of infection, and false-negative results for very high falciparum parasitaemias and parasites with mutated/deleted hrp2 and hrp3 genes [10, 43]. This evidence concerns the gene HDGFL3 and malaria.