In conclusion, a total of 15 hub genes (CCL5, CCR7, CD2, CD27, CD274, CD3D, GNLY, GZMA, GZMH, GZMK, IL2RB, IL7R, ITK, KLRB1, and PRF1) have been screened out as sepsis biomarkers in this study, all of them were downregulated genes in sepsis. The gene discussed is PRF1; the disease is Sepsis.