Based on the above marker genes of subpopulations, ssGSEA analysis was used to calculate score of the two hub subpopulations, and variance analysis of subpopulations score between osteoporosis and no-osteoporosis indicated that CD45+ BM-MSCs_5 subpopulation had significant difference (Figures 2(d) and 2(e)). This evidence concerns the gene PTPRC and osteoporosis.