First, we overexpressed GSDMC in PANC-1 and CFPAC-1 cells, the expression of Ki-67 and vimentin was significantly increased and the expression of E-cadherin was significantly reduced, which indicated that overexpression GSDMC can promote PAAD cell proliferation and invasion (Additional file 9: Fig. S9A). This evidence concerns the gene MKI67 and pancreatic adenocarcinoma.