Moreover, compared to WT controls, OL clusters with increased ERK/MAPK and SPP1 transcriptome in single cell RNAseq data were detected from the microglial Nhe1 cKO white matter brain tissues after stroke (data not shown), which further support role of microglial NHE1 protein in microglia–OL communications. The gene discussed is SLC9A1; the disease is stroke disorder.