To confirm that lnc-SELPLG-2:1 regulates osteosarcoma via the lnc-SELPLG-2:1/hsa-miR-10a-5p /BTRC axis, the cell function of pcDNA3.1-lnc-SELPLG-2:1 was validated by transfection with hsa-miR-10a-5p mimics. The gene discussed is BTRC; the disease is osteosarcoma.