In order to determine which lncRNA was involved in osteosarcoma, lncRNAs including lnc-SELPLG-2:1, lnc-DDX47–3:1, lnc-ZNF77–165:3, lnc-SRSF2–9:2, lnc-SAMD11–1:1, and lnc-PPP1R9B-4:2 were detected by RT-qPCR to determine if their sequences were consistent with sequencing results. The gene discussed is SELPLG; the disease is osteosarcoma.