Co-transfection of pcDNA3.1-lnc-SELPLG-2:1 and hsa-miR-10a-5p mimics decreased cell proliferation, migration, and invasion ability, and increased apoptosis and hsa-miR-10a-5p reversed the osteosarcoma-promoting effect of lnc-SELPLG-2:1. The gene discussed is SELPLG; the disease is osteosarcoma.