Mass spectrometry (MS) analysis of purified PKM2 from in vitro assays (Fig. 2B) and in cultured H1299 cells (Fig. 2C) further revealed Aurora A phosphorylated PKM2 at Threonine 45 (T45), an evolutionarily conserved residue (Fig. S1A) that was also phosphorylated by Aurora B kinase during cytokinesis in glioblastoma cells (19). Here, AURKB is linked to glioblastoma.