Pygo2 rs141722381 leads to damage to the tertiary structure of protein and rs61758741 is a missense mutation that replaces a basic amino acid with an acidic amino acid.29 It is reported that 2 SNP mutations in PYGO2 (rs61758740 and rs141722381) are correlated to idiopathic azoospermia.6 Moud et al5 have also reported that there is a relationship between PYGO2 rs61758740 and male infertility among the Iranian population, but there is no significant correlation between rs61758741 and male infertility5 which confirms our findings. This evidence concerns the gene PYGO2 and male infertility.