We generated SARS-CoV-2 Spike-pseudotyped lentivirus carrying ZsGreen and luciferase reporters to determine if ZMPSTE24 depletion influenced infection of HEK293T cells that had been stably transduced with ACE2 (or ACE2-mCherry), which encodes the SARS-CoV-2 receptor (Fig. 2A). The gene discussed is ACE2; the disease is infection.