As a starting point in this study and to verify the published results for the protective role of ZMPSTE24 in human cell lines, we produced lentivirus pseudotyped with vesicular stomatitis virus glycoprotein (VSV-G) that carried two reporter genes, ZsGreen to visualize infected cells by fluorescence microscopy and luciferase to quantitate infection (Fig. 1A). The gene discussed is ZMPSTE24; the disease is infection.