Briefly, CFSE-labeled THP-1 AML cells engineered to stably overexpress TIM-3 (THP-1/TIM-3-Flag) and CTV-labeled THP-1 parental cells were co-cultured with purified T cells in the presence of anti-CD3/anti-CD28 T-cell activation beads and 25 μg/ml sabatolimab whole antibody, sabatolimab Fab or hIgG4 isotype control. The gene discussed is CD28; the disease is acute myeloid leukemia.