When analyzing GP73 and NF-κB in two hepatoma cell lines (HepG2 and Huh7) transfected with pHBV1.3, we found that GP73 mRNA levels positively correlated with pHBV1.3 expression, and NF-κB mRNA levels negatively correlated with pHBV1.3 expression (Fig. 7A). The gene discussed is NFKB1; the disease is hepatocellular carcinoma.