First, we compared the internal areas of the primary tumor and liver metastases, found that 8 markers (CD8, Foxp3, CD68, CD163, CD20, CD11c, VEGFR-2, PD-L1) had higher expression in TF than TC region in both primary tumor and liver metastases (p < 0.05). This evidence concerns the gene FOXP3 and neoplasm.