In order to know whether hsa_circ_0003176 participated in the regulation of NSCLC cell proliferation, migration, and invasion by regulating RBM5 expression, the designed RBM5 shRNA (shRNA#1, shRNA#2, and shRNA#3) was used to silence the expression of RBM5 in both A549 and H1299 cells. Here, RBM5 is linked to non-small cell lung carcinoma.