RBM5 and non-small cell lung carcinoma: Subsequently, the NSCLC cell lines (A549 and H1299) were transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid, and RT-qPCR and western blot assay showed that transfection of sh-RBM5 plasmid can reduce the mRNA and protein expression of RBM5 in A549 and H1299 cells overexpressing hsa_circ_0003176 (Figures 7(a) and 7(b)).