CASP3 and hepatocellular carcinoma: The apoptosis of cells was detected by flow cytometry, i.e., the apoptosis rate was higher in the si-SIRT group than in the si-Ctrl group, so it can be indicated that inhibition of the SIRT1 gene can promote apoptosis of hepatocellular carcinoma cells, and this conclusion was verified by protein immunoblotting with increased levels of apoptosis-related caspase-3 and PARP.