Beclin-1 and LC3II protein levels in heart tissue of chronic HF patients presented elevation, and both of them presented downregulation after application of left ventricular assist device [30]; in HF mice induced by adriamycin, a large number of autophagosome structures were observed [31]; Nakai et al. specifically knocked down autophagy gene ATG5 in mice, and then cardiac function of mice presented depletion [32]. This evidence concerns the gene BECN1 and hydrops fetalis.