ACOT2 and acute myeloid leukemia: To gain further biological insights into the underlying mechanisms of ACOT2 overexpression in AML, GSEA analysis revealed that the gene sets were significantly enriched in estrogen response early (NES = 1.479, P=0.046) (Figure 5(b)), heme metabolism (NES = 1.994, P=0.046) (Figure 5(c)), interferon alpha response (NES = 1.567, P=0.039) (Figure 5(d)), myogenesis (NES = 1.504, P=0.046) (Figure 5(e)).