qRT-PCR results demonstrated that the expression of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and osteopontin-1 (OPN-1) were significantly upregulated in the TSD group compared with the control serum group (Figure 5(a), P < 0.001). The gene discussed is OPHN1; the disease is Tay-Sachs disease.