ATG5 and osteoporosis: Moreover, selective deletion of autophagy-related gene 5 (ATG5) in osteoclasts in ATG5flox/flox-LyzM-Cre+ mice reduce about 50% bone loss in ovariectomy-induced osteoporosis and the Atg5, Atg7, Atg4B, and LC3 proteins in autophagy were important for generating the osteoclast ruffled border, the secretory function of osteoclasts and bone resorption bone resorption in vitro and in vivo. Furthermore, Rab7, which is required for osteoclast function, localizes to the ruffled border in an Atg5-dependent manner.