We herein analyzed several markers of ferroptosis—including the GSH/GSSG ratio; the levels of MDA, MPO, 4-HNE, GPX4, and ACSL4; and the mitochondrial damage noted in several mouse AKI models—and our data revealed that the abnormal changes observed in the renal tissues of AKI mice were robustly ameliorated with SKQ1 treatment. The gene discussed is ACSL4; the disease is acute kidney injury.