Finally, we found that HSC-derived HK1 can also influence extrahepatic metastasis of HCC, because lEVs derived from control Hepa1-6 cells but not those derived from HK1 knockdown Hepa1-6 cells significantly promoted lung tumor metastasis (Fig. 4o), with elevated expression of HK1 in the metastatic tissues (Fig. 4p). The gene discussed is HK1; the disease is hepatocellular carcinoma.