To investigate whether endogenous TRIM11 regulates KDM5C, we knocked down TRIM11 with different siRNAs in multiple cell lines, including breast cancer cell lines MDA-MB-231 and MDA-MB-468, kidney cell lines HEK293T, 769-P, 786-O and OS-RC-2, liver cell lines HepG2, HL7702 and Li-7, colon cell lines HCT15 and SW480, prostate cell lines PC3, DU145, 22RV1 and PNT1A, cervix cancer cell line HeLa, bladder cell lines EJ, SV-HUC-1 and UMUC3, and osteosarcoma cell line U2OS. The gene discussed is TRIM11; the disease is breast cancer.