CD274 and cancer: To understand the potential biological consequence of PD-L1 upregulation by MLN4924, we asked whether MLN4924 induction of PD-L1 in cancer cells would negatively affect the viability and function of T cells, and performed a co-culture assay of the Jurkat T cells with cancer cells with or without MLN4924 pretreatment (Figs. 4A and S5A, top panel).