To determine whether this is a general phenomenon, we extended the observation and found that MLN4924 increased the levels of PD-L1 protein in multiple human cancer cell lines, including esophageal and head & neck cancer cells (Kyse70, UMSCC1, UMSCC38, UMSCC5, UMSCC10, UMSCC11b, UMSCC17); pancreatic cancer cells (Moh1, MIAPaCa2, BxPC3) and lung cancer cells (H358, H1792, H460, A549, H1650, H1299) (Fig. S1A–E), as well as few murine cancer cell lines, B16-F10, MC38 and CT26 (Fig. S1F), indicating a general phenomenon. Here, CD274 is linked to familial pancreatic carcinoma.