Furthermore, Molecular Signature Database (MSigDB) focused on hallmark gene sets (see Methods) scored the enrichment of 4 hallmarks related to cancer proliferation (i.e., E2F targets, EMT, G2M checkpoint and mitotic spindle) both in HeyA8 and in ES-2 cell lines (Fig 5B and 5C), which is in line with previously described enhanced proliferation and migration of HeyA8 and ES-2 cells upon ZNF521 overexpression (Figs 2 and 3). Here, ZNF521 is linked to cancer.