However, this amplification covers over 4 MB and contains nearly 50 genes (Sinclair et al., 2003) including Tbx2, PRKCA, Tlk2, TUBD1, and PPM1D, that are known for their correlation to breast cancer and/or contribution to oncogenic signaling (Sinclair et al., 2003; Olson et al., 2011; Abrahams et al., 2008; Lu et al., 2005; Ali et al., 2012; Groth et al., 2003; Xu et al., 2008; Monni et al., 2001), making estimation of the contribution of S6K1 amplification to the disease difficult. This evidence concerns the gene RPS6KB1 and breast cancer.