Using clinical sepsis diagnosis as the “gold standard.” ALB had an area under ROC curve of 0.728 (95% CI: 0.6190~0.820), 58.06% sensitivity and 80.95% specificity (optimal cutoff value:30.2g/L), while NGS had an area under the ROC curve of 0.857 (95% CI:0.763~0.924),95.16% and 76.19%. The gene discussed is ALB; the disease is Sepsis.