In the present study, we performed a comparative analysis of the prevalence of anti-SARS-CoV-2 IgG antibodies against the S1 subunit of the spike glycoprotein and the factors potentially associated with infection after the first and second waves of COVID-19 in the city of Belém, the capital of the state of Pará, the largest metropolis in the Brazilian Amazon. Here, PSMD1 is linked to infection.