Recommendations for future studies include evaluation of additional cytokine release assays such as interferon gamma-induced protein 10 (IP-10), MIG, tumour necrosis factor alpha (TNF-α), interleukin-2 (IL-2) used in biosignatures in human TB, as well as circulating serum biomarkers that could be incorporated into a diagnostic algorithm for felids using a convenient set of samples (Yong et al., 2019; Morris et al., 2021). Here, TNF is linked to tuberculosis.