GSDMD and neoplasm: To investigate the immunological characteristics of pyroptotic tumor cells and their potency to be developed as a personalized tumor vaccine, we explored a generally applicable strategy for inducing pyroptosis of tumor cells utilizing the inducible Tet-On systems for the expression of the key downstream effector protein GSDMD-NT (1~276 a.a.)(9, 28, 29, 34), which is independent of any upstream receptor recognition or caspase activation.