By integrating serum monoclonal IgA and IgG from patients with multiple myeloma preparation in the HYDRASYS system and in-agarose-gel digestion without intact N-glycopeptide enrichment, we compared the identification performance of four different mass spectrometry dissociation methods (EThcD-sceHCD, sceHCD, EThcD and sceHCD-pd-ETD). The gene discussed is CD79A; the disease is plasma cell myeloma.