Although determination of TREC in newborn screening for SCID has proven high sensitivity, it should be stressed that in order not to miss any other types of PID as agammaglobulinemia, Nijmegen breakage syndrome, AT or DiGeorge syndrome, as well as late-onset ADA SCID, newborn screening for inborn errors of immunity in the form of triple assay (including TREC and KREC) is optimal. This evidence concerns the gene ADA and 22q11.2 deletion syndrome.