Endothelial function (plethysmography), hyperaemia index, HR and augmentation index, BP, multiple biomarkers (Pro-adrenomedullin, Pro-atrial natriuretic peptide, inter leukin 6, Tumour necrosis factor, ICAM-1 protein, VCAM-1 Biomarker, vascular endothelial growth factor. BMI. Multidimensional Fatigue Inventory (MFI-20 questionnaire), Major Depression Inventory (MDI), World Health Organisation Five well-being (WHO-5), Chronic stress Ull-meter, Montreal Cognitive Assessment, Metabolic Equivalent of Task (MET) calculations from activity and HR measures,. The gene discussed is NPPA; the disease is major depressive disorder.