In order to further study the molecular mechanisms of apatinib combined with WZB117 inhibiting glycolysis level of melanoma cells, we detected the expression of key glycolysis kinases HK2, PFKFB3, PKM2, and regulatory factor HIF-1α in A375 cells and SK-MEL-28 cells treated with apatinib or WZB117 alone or in combination for 48 h. The gene discussed is HIF1A; the disease is melanoma.