In particular pRNFL correlated with global and regional brain atrophy (17–19, 23, 24), with MRI lesion load (23, 35), MTR measures (36), with NAA/Cho ratio in proton brain MRI spectroscopy (37), with cerebrospinal fluid neurofilament light chain levels (38, 39), neurofilament light chain levels in serum (40), EDSS progression (41), cognitive decline (42) and disease-modifying treatment (DMT) response (43). This evidence concerns the gene NEFL and Brain atrophy.