We found that the GFP fluorescence was significantly reduced in nphs1-/- in both the retinal vessel plexus of eye and the dorsal aorta at 8 dpf compared to the wild-type siblings (Figure 4K), indicating that nphs1 mutations led to loss of VDBP-GFP due to leaky GFB, a phenotype resembling hypoalbuminemia seen in NS patients. This evidence concerns the gene NPHS1 and Hypoalbuminemia.