Although adult male C57BL/6 mice are frequently used in the study of TN and related emotions (Trevisan et al., 2016; Chen et al., 2021) and we also provided preliminary evidence demonstrating that the LHb plays an important role in the regulation of TN and related anxiety-like behaviors using these mice (Cui et al., 2020b), whether our findings differs according to species and sex requires further validation. The gene discussed is LHB; the disease is Anxiety.