To determine whether snake venom induces autophagy in other cancer cell lines, we confirmed the efficacy of snake venom against A549 lung cancer cells, HeLa CCL2 cervical cancer cells, and U-2 OS osteosarcoma cells; snake venom treatment increased the level of LC3-II and LC3 puncta in some of these cancer cell lines (Supplementary Figure S2A and S2B). Here, MAP1LC3A is linked to osteosarcoma.