Then, 9 target genes that were differentially expressed between bladder cancer and normal samples were screened (FDR < 0.05, Supplementary Figure 3), in which upregulated expression of RHBG, PAQR6, CLK2, KRTCAP2, FLAD1, HCN3 were correlated with bad survival of patients with bladder cancer (Supplementary Figure 4). The gene discussed is HCN3; the disease is urinary bladder cancer.