Three genes including integrin subunit Beta 1 (ITGB1), melanoma cell adhesion molecule (MCAM), and protein phosphatase 1 regulatory subunit 16A (PPP1R16A) highly expressed in HCC and negatively correlated with patient survival were further identified (Figure 4A and Figure 4B). The gene discussed is PPP1R16A; the disease is hepatocellular carcinoma.