Next, we further measured the caspase-3 and caspase-8 expressions in the colon of DSS-induced mice with or without TSPs treatment by immunohistochemical staining, which showed clear increased staining in the colonic mucosa of DSS-induced colitis mice compared with controls, while TSPs intervention largely reversed this trend (Figures 7C–F). Here, CASP8 is linked to colitis.