Next, the western blot and qRT-PCR results revealed that compared to the other three groups, protein level and mRNA level of phosph-STAT1, the target in the IFNγ and STAT3 inhibitor combination group were the highest in NSCLC cells (H1299, H1975, PC9, H3255). The gene discussed is IFNG; the disease is non-small cell lung carcinoma.