First, we downloaded and analyzed the expression profiles of 504 HNSCC and 44 normal cases and found that 69 genes, including GPX4, BID, PHKG2, ULK1, ATG13, TFRC, ATG3, CARS1, SLC1A5, SOCS1, MAPK8, TFR2, and others, were significantly highly expressed (p < 0.05); TF, PRKAA2, CDO1, PEBP1, LPIN1, and other 15 genes were significantly expressed at low levels (p < 0.05, Figure 1A); the expression of other ferroptosis-related genes were not significantly different between the tissues (p > 0.05, Supplementary Table S1); we found a similar trend in the GSE12452 dataset (Figure 1B). This evidence concerns the gene LPIN1 and head and neck squamous cell carcinoma.