Effector genes such as GZMB, GZMH and KLRG1 were the marker genes in clonotypes shared by all tissues, while the clonotypes present only in tumor showed upregulated T-cell exhaustion genes (HAVCR2, LAG3, CTLA4, TIGIT, PDCD1, and ICOS) and activation genes (SELL and TNFRSF9) (Figure 5E). This evidence concerns the gene ICOS and neoplasm.