Collectively, these data point to the presence of two distinct populations of expanded T cells in tumor: 1), a Teff population expressing a “go” signal and interstitial migration genes with large clonal size refreshed from T cells in the circulation, and 2), a Tem population with the TCRs only present in tumor, expressing markers of antigen experienced markers and exhaustion genes, and high expression of RHOH as an inhibitory signal for chemokine mediated migration. This evidence concerns the gene RHOH and neoplasm.