To confirm the association between TRAF3IP3 and the ERK pathway and to identify whether the ERK pathway is involved in TRAF3IP3-mediated glioma proliferation, migration, and invasion in vitro, U118 cells transfected with the PCDH/TRAF3IP3 vector were treated with or without the ERK pathway inhibitor U0126. Here, TRAF3IP3 is linked to central nervous system cancer.