The eukaryotic initiation factor eIF-5A2 has been reported to be an oncogenic protein in multiple human cancers.233–236 Accumulated evidence suggests that eIF-5A2 initiates tumor formation, enhances cancer cell growth, and increases cancer cell metastasis.146,237,238 Guan XY et al. showed that eIF-5A2 is amplified in the ovarian cancer cell line UACC-1598 in the form of DMs.146 Conclusively, amplification of oncogenes on eccDNA provides a novel theoretical basis for explaining tumorigenicity. The gene discussed is EIF5A2; the disease is cancer.