Taking in consideration the impact on HS biosynthesis observed in the glycoengineered cell models and knowing that Syndecans (SDCs) and glycosylphosphatidylinositol-anchored (GPI)–anchored Glypicans (GPCs) are major carriers of HS on epithelial cells, we then evaluated the impact of EXTL2 and EXTL3 KO over SDCs and GPCs expression in the gastric cancer cells. The gene discussed is GPC1; the disease is gastric cancer.