As shown in Figure 3, low expression of HAND2-AS1 was related to poor tumor differentiation (OR = 4.36, 95% CI: 2.15–8.87, P < .001) and lymph node metastasis (OR = 0.26, 95% CI: 0.13–0.54, P < .001). This evidence concerns the gene HAND2 and metastatic malignant neoplasm in the lymph nodes.