KM analysis showed that the clinical features, including alpha-fetoprotein (AFP) (P = .02871), stage (P = 3e − 05), T (P = 2e − 05), N (lymph node) (P = .02519), and M (metastasis) (P = .00223) could divide the HCC patients of training set based on OS analysis (Figure S5, Supplemental Digital Content 9, http://links.lww.com/MD/H374). The gene discussed is AFP; the disease is hepatocellular carcinoma.