The distribution of the top 20 mutated genes in GBM is illustrated in Fig. 4D. Furthermore, we compared the genes mutations between GBM in the high- and low- risk groups, and the results showed that the mutation frequency of IDH1, SYNE1, ADAM29, CACNA2D1, DICER1, and USH2A in GBM in the low-risk group was significantly higher than that in GBM in the high-risk group (Fig. 4E and F). This evidence concerns the gene CACNA2D1 and glioblastoma.