Consistent with the findings of RNAseq analysis, transcript expression of IFNG, IL1B, IL4, IL8, and IL13 as detected by qRT-PCR remained unaltered in both bro-ALI and alv-ALI exposed to HTP-smoke compared to the corresponding sham (Supplementary Fig. S2). The gene discussed is CXCL8; the disease is acute respiratory distress syndrome.