VCP and amyotrophic lateral sclerosis: The objective of this study was twofold: (1) to deepen our understanding of the role(s) of cytoplasmic IR in normal cellular physiology by resolving the spatiotemporal dynamics of IRTs underlying distinct stages of MN lineage restriction and (2) to decipher whether specific classes of IRTs become dysregulated in the context of disease by systematically examining the influence of ALS-causing VCP mutations on this process.